補益中藥對運動性腎缺血再灌注損傷大鼠腎臟炎癥因子及 ECM 表達的影響
鄭孟君1 ,曹建民2 ,郭 嫻2 ,周海濤3
(1. 江南大學 體育學院,江蘇 無錫 214000;2. 北京體育大學 運動人體科學學院,北京 100084;
3. 北京聯合大學 生物化學工程學院,北京 100023)
摘 要: 觀察補(bu)益中藥(yao)對運(yun)動(dong)性(xing)腎(shen)缺血再灌(guan)注(zhu)損傷(shang)大鼠(shu)腎(shen)臟炎癥因子及 ECM 表(biao)達的影響。方法:75 只(zhi) 7 周齡雄性(xing)Wistar 大鼠(shu)隨(sui)機(ji)分為 4 組(zu)(zu)(zu)(zu)(zu):安靜對照(zhao)組(zu)(zu)(zu)(zu)(zu)( C 組(zu)(zu)(zu)(zu)(zu),12 只(zhi)) 、一般訓練(lian)組(zu)(zu)(zu)(zu)(zu)( M 組(zu)(zu)(zu)(zu)(zu),12 只(zhi)) 、過度(du)訓練(lian)組(zu)(zu)(zu)(zu)(zu)( OM 組(zu)(zu)(zu)(zu)(zu),24 只(zhi)) 和補(bu)益中藥(yao) + 過度(du)訓練(lian)組(zu)(zu)(zu)(zu)(zu)( TM 組(zu)(zu)(zu)(zu)(zu),24 只(zhi)) ,M、OM、TM 組(zu)(zu)(zu)(zu)(zu)進行 8 周 56 天的游泳訓練(lian)。采用(yong)專(zhuan)業灌(guan)胃器每天灌(guan)胃 1 次(ci),TM 組(zu)(zu)(zu)(zu)(zu)灌(guan)胃采用(yong)黃(huang)芪、紅景天、丹參、苦(ku)參等(deng)組(zu)(zu)(zu)(zu)(zu)成的復合中藥(yao)制劑(ji),劑(ji)量為 1 g·kg - 1 ,體積為 5 mL·kg - 1 ,其他各組(zu)(zu)(zu)(zu)(zu)灌(guan)胃等(deng)量生(sheng)理鹽水。末次(ci)訓練(lian)后 24 h,采用(yong) PAS 染色觀察腎(shen)小球(qiu) ECM 沉(chen)積情況(kuang),免(mian)疫組(zu)(zu)(zu)(zu)(zu)織(zhi)化學法檢(jian)測(ce)腎(shen)組(zu)(zu)(zu)(zu)(zu)織(zhi) TNF-α、IL- 1β、IL-6、IL-18 蛋白表(biao)達,RT-PCR 法檢(jian)測(ce)腎(shen)組(zu)(zu)(zu)(zu)(zu)織(zhi) TNF-α mRNA、IL-1β mRNA、IL-6 mRNA、IL-18 mRNA、TGF-β1 mRNA表(biao)達。結果:1)8 周實驗后,腎(shen)小球(qiu) ECM 沉(chen)積,C、M 組(zu)(zu)(zu)(zu)(zu)間無顯(xian)著差(cha)異( P > 0. 05 );OM 組(zu)(zu)(zu)(zu)(zu)較(jiao) C、M 組(zu)(zu)(zu)(zu)(zu)顯(xian)著增加( P <
0. 01);TM 組(zu)(zu)顯著(zhu)低于 OM 組(zu)(zu)( P < 0. 05)。2) 血尿素氮和血清肌酐(gan)水(shui)平,OM 組(zu)(zu)和 TM 組(zu)(zu)顯著(zhu)高于 C 組(zu)(zu)( P < 0. 01), TM 組(zu)(zu)顯著(zhu)低于 OM 組(zu)(zu)( P < 0. 05);腎組(zu)(zu)織(zhi) TNF-α、IL-1β、IL-6 和 IL-18 蛋白表達,OM 組(zu)(zu)和 TM 組(zu)(zu)顯著(zhu)高于 C 組(zu)(zu)( P <
0. 01) ,TM 組(zu)(zu)(zu)(zu)(zu)(zu)顯(xian)著(zhu)低于 OM 組(zu)(zu)(zu)(zu)(zu)(zu)( P < 0. 05);腎(shen)組(zu)(zu)(zu)(zu)(zu)(zu)織 TNF-α mRNA、IL-1β mRNA、IL-6 mRNA 和(he)(he) IL-18 mRNA 表(biao)達(da),OM、 TM 組(zu)(zu)(zu)(zu)(zu)(zu)顯(xian)著(zhu)高(gao)于 C 組(zu)(zu)(zu)(zu)(zu)(zu)( P < 0. 01) ,TM 組(zu)(zu)(zu)(zu)(zu)(zu)顯(xian)著(zhu)低于 OM 組(zu)(zu)(zu)(zu)(zu)(zu)( P < 0. 05);腎(shen)組(zu)(zu)(zu)(zu)(zu)(zu)織 TGF-β1mRNA 表(biao)達(da),TM 組(zu)(zu)(zu)(zu)(zu)(zu)( P < 0. 05) 和(he)(he) OM 組(zu)(zu)(zu)(zu)(zu)(zu)( P < 0. 01) 顯(xian)著(zhu)高(gao)于C 組(zu)(zu)(zu)(zu)(zu)(zu),TM 組(zu)(zu)(zu)(zu)(zu)(zu)顯(xian)著(zhu)低于OM 組(zu)(zu)(zu)(zu)(zu)(zu)( P < 0. 05)。結論:8 周過度(du)訓練(lian)致大(da)鼠(shu)發生運動性腎(shen)缺(que)血(xue)再(zai)灌注(zhu),同(tong)時 ECM 沉(chen)積加強。補充補益中藥(yao)可通過抑制腎(shen)臟(zang)(zang)組(zu)(zu)(zu)(zu)(zu)(zu)織 TNF-α、IL-1β、IL-6、IL-18 的(de)(de)(de)(de)表(biao)達(da)進(jin)(jin)而(er)減輕(qing)了過度(du)訓練(lian)誘導腎(shen)臟(zang)(zang)缺(que)血(xue)再(zai)灌注(zhu)發生時腎(shen)臟(zang)(zang)組(zu)(zu)(zu)(zu)(zu)(zu)織 TGF-β1 的(de)(de)(de)(de)表(biao)達(da),抑制 ECM 的(de)(de)(de)(de)合成和(he)(he)( 或(huo)) 促進(jin)(jin) ECM 的(de)(de)(de)(de)降解及組(zu)(zu)(zu)(zu)(zu)(zu)織病理學改變,延(yan)緩(huan)或(huo)避免過度(du)訓練(lian)導致的(de)(de)(de)(de)運動性缺(que)血(xue)再(zai)灌注(zhu)對腎(shen)臟(zang)(zang)的(de)(de)(de)(de)損害(hai)。
關鍵詞: 補益(yi)中藥;運動性腎(shen)缺血再灌注損傷;炎癥因子;細(xi)胞外基質
中圖分類號: G804. 7 文(wen)獻標志碼(ma): A 文(wen)章編號: 1004 - 0560( 2015) 03 - 0068 - 06
Effect of Tonic Medicine on Kidney Inflammation Factors and ECM Expression in Rats of Exercise-related Renal Ischemia-reperfusion Injuries
ZHENG Mengjun1 ,CAO Jianmin2 ,GUO Xian2 ,ZHOU Haitao3
(1. Sports College,Jiangnan University ,Wuxi 214000,Jiangsu,China;
2. Sport Science College of Beijing Sport University ,Beijing 100084,China;
3. Biochemical Engineering College of Beijing Union University ,Beijing 100023,China)
Abstract:Objective:To study the protective effects of tonic medicine on kidney inflammation factors and extracellular ma- trix ( ECM ) expression in rats of exercise-related renal ischemia-reperfusion injuries. Methods:75 male Wistar rats at the age of 7 weeks were divided into 4 groups randomly:control group( C group,n = 12) ,general training group( M group,n =
12) ,overtraining group( OM group,n = 24) ,tonic medicine + overtraining group( TM group,n = 24) ,the Rats in M , OM ,TM groups were assigned to 8 weeks of swimming training,7 days a week. Professional gavage were given once a day. The Rats of TM group were given with compound Chinese herbal medicine( The primary elements were Chinese drugs for invigoration,such as Astragalus,Rhodiola rosea,Salviae miltiorrhizae,Sophora flavescens) ,gavages( ig) dose was 1 g
· kg - 1 ;ig volume was 5 mL·kg - 1 ,and other groups were given the normal saline. 24 hours after the last training,w e ob-
served the glomerular ECM deposition by the PAS stain;detected the renal tissue of TNF-α,IL-1β,IL-6,IL-18 protein by immunohistochemistry and detected the expression of TNF-α mRNA,IL-1β mRNA,IL-6 mRNA,IL-18 mRNA,TGF-β1 mRNA gene in renal tissue by RT-PCR method. Results:1)8 weeks after the experiment,the glomerular ECM deposition of C group and M group appeared normal and showed no differences( P > 0. 05);OM group was significantly higher than that in C group and M group( P < 0. 01) ,TM group was significantly lower than that of group OM ( P < 0. 05). 2) OM
收稿日期(qi): 2015-03-04; 修回(hui)日期(qi): 2015-03-29
基金項目: 國家體(ti)育(yu)總(zong)局課題(2013A101)。
作者(zhe)簡介: 鄭孟(meng)君(jun)(1980—) ,女,講(jiang)師,碩士,主要研究方向(xiang)(xiang)為(wei)(wei)體育教學與(yu)運動訓練。通信作者(zhe): 周海濤(1976—) ,男,副教授,碩士,主要研究方向(xiang)(xiang)為(wei)(wei)運動性(xing)疲勞與(yu)恢(hui)復。
第(di) 3 期 鄭孟君,等: 補益中藥對運動性(xing)腎缺(que)血再(zai)灌注損(sun)傷大鼠腎臟炎癥因子及 ECM 表(biao)達的影響 ·69·
group and TM group blood urea nitrogen and serum creatinine were significantly higher than that of C group( P < 0. 01), TM group was significantly lower than that of group OM ( P < 0. 05);the expression of renal TNF-α,IL-1 β,IL-6 and IL- 18 protein in OM group and TM group was significantly higher than that in C group( P < 0. 01) ,TM group was significant- ly lower than that of group OM ( P < 0. 05);the expression of renal TNF- α mRNA,IL-1 β mRNA,IL-6 and IL-18 mRNA in TM group and OM group was significantly higher than that of C group( P < 0. 01) ,TM group was lower than of OM group( P < 0. 05);the expression of renal TGF-β1mRNA in TM group and OM group was significantly higher than that of C group( TM ,P < 0. 05;OM ,P < 0. 01) ,TM group was significantly lower than of OM group( P < 0. 05). Conclusion: 8 weeks overtraining caused the movement of rat renal ischemia reperfusion and increased the deposition of ECM . Tonic medicine can restrain the expression of renal tissue TGF-β1 during exercise-related renal ischemia-reperfusion injury in- duced by overtraining,w hich may be achieved by inhibiting the expression of IL-1,TNF-α,IL-6,IL-18 in renal tissue. Fi- nally it decreased the synthetic and increased the decomposition of ECM ,reduced the damage and the histopathological changes,as a result,Tonic medicine can protect the rats from exercise-related renal ischemia-reperfusion injury.
Key words:tonic medicine;exercise-related renal ischemia-reperfusion injury;inflammation factors;extracellular matrix
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